Genomics

If you need to book instrument use, training, or consulation please email: Mbcf@etsu.edu

Genomics covers a broad range of molecular techniques spanning the study of structure, function, evolution, mapping, and editing of genomes. Your core offers a number of technologies and services to meet your experimental needs.

• Genotyping

  
  From snip to report

  Simply provide us with your sample (2-3 mm tail snips or ear punches), primers, and a description of your validated PCR assay conditions.  The Core Facility will then perform all the steps of the genotyping assay and send your report via email. The normal turn-around time for this service is two business days. The cost for the full genotyping service is $9/sample, competitive with outside vendors, PLUS there are no FedEx shipping fees.

  DNA extraction only: For investigators who wish to do their own PCR reactions, but do not wish to expend valuable technician time and reagent costs for routine isolation of DNA, the Core Facility offers genomic DNA isolation services for $3/sample. the normal turn-around period for this service is one business day.

• Capillary Sequencing

  Using a Beckman CEQ 8000 automated DNA sequencer, your core can get up to 800 bp of readable DNA sequence from plasmids, PCR products, phage or cosmid clones. Sequencing larger templates (BACs, YACs, etc.) is also possible. In combination with WellRed chemistry the facility can produce excellent DNA sequence data at a lower cost than using manual gels and radioactive labeling. Standard primers (T7, T3, SP6, M13F, M13R, etc.) are provided for free. Other primers are provided by user at 3 uM. Primer design services are available. Sequenced data are returned to the user by email generally in 1-2 business days. 

  See more details about sample requirements and download submission forms.

• Next-Generation Sequencing

  Your core has partnered with the UT Genomics Core at the University of Tennessee Knoxville for access to an Illumina MiSeq high-throughput Next-Gen sequencer. The MiSeq platform  provides up to 15 Gb of DNA sequence in a single run. Applications include genome sequencing, RNA sequencing, metagenomics, and 16S metabarcoding, as well as custom amplicon sequencing. 

   

• qPCR

  Real-time PCR, also called qPCR, RT-PCR, or kinetic PCR,  is widely used in gene expression analysis either as a primary method or as a confirmation of other methods such as microarray analysis. Real-time PCR is also the most commonly used method to monitor the efficiency of gene silencing by RNA interference. Other applications include measurement of DNA copy number and transgene copy number, determination of viral titers, and single nucleotide polymorphism genotyping.

  Unlike conventional PCR that uses an end-point analysis of the amplicon, real-time PCR is based on the detection and quantitation of the PCR product as it is made, using either fluorogenic specific probes (such as Taqman probes) or double-stranded DNA binding fluorescent dyes such as SYBR Green.

• Primer Design

  Your core personnel can assist with primer design for PCR, qPCR, or sequencing. Contact us with details about your primer needs.